Figure 2.

The Effect of INHBA-AS1 on Invasion, Migration, and Proliferation in Trophoblast Cell Line

(A and B) Invasion and migration of HTR-8/SVneo cells were detected by a Transwell assay after INHBA-AS1 overexpression (A) or knockout (B). Upper panel: pictures of cells stained with 0.1% crystal violet solution; lower panel: statistics of cells. Scale bars, 100 μm. (C and D) The proliferation of HTR-8/SVneo cells was detected by an EdU assay after INHBA-AS1 overexpression (C) or knockout (D). Upper panel: pictures of cells stained with 4′,6-diamidino-2-phenylindole (DAPI) and EdU; lower panel: statistics of EdU-positive cells. (E and F) Cell Counting Kit-8 was used to evaluate the cell proliferation of HTR-8/SVneo cells after INHBA-AS1 overexpression (E) or knockout (F). LV, lentivirus; NC, normal control. The values are shown as the mean ± SD of three independent experiments; ∗∗p < 0.01, ∗p < 0.05. See also Figure S1.