Figure 3.

INHBA-AS1 Prohibits Cell Cycle and Promotes Apoptosis in HTR8/SVneo Cells

(A) Cell-cycle analysis by flow cytometry on cells transfected by LV harboring human full-length INHBA-AS1 or empty vector. Upper panels: frequency histograms of cell-cycle phases; lower panels: statistics of cell-cycle phases. (B) Cell-cycle analysis by flow cytometry on cells with INHBA-AS1 knockout. Upper panels: frequency histograms of cell-cycle phases; lower panels: statistics of cell-cycle phases. (C and D) Apoptosis analysis using Annexin-V assay coped with flow cytometry on cells with INHBA-AS1 overexpression (C) or knockout (D). Upper panels: histograms of apoptotic cells; lower panels: statistics of apoptotic cells. The apoptotic cells include cells in quad 2 (Q2) and Q4. Cells in Q2 are dead apoptotic cells (PI positive and Annexin-V positive), and cells in Q4 are ongoing apoptotic cells (PI negative but Annexin-V positive). The values are shown as the mean ± SD of three independent experiments; ∗∗p < 0.01, ∗p < 0.05. See also Figure S1.