Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Dec 16;40(11):2188–2200. doi: 10.1177/0271678X19891839

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2019

PMC Copyright notice

Figure 1. — LPS/HI triggers rapid inflammatory responses in neonatal mouse brains. (a) RT-qPCR analysis suggested elevated expression of multiple pro-inflammatory and neutrophil/monocyte-recruiting cytokines in the ipsilateral (R) hemisphere at 6 h after HI or LPS/HI injury compared with unchallenged (UN), LPS-injected, or the contralateral (L) hemisphere, although the cytokine induction patterns by HI-versus-LPS/HI were not identical. Of note, LPS/HI injury is far more potent for inducing the Csf3 mRNA. (b, c) Detection of leukocyte infiltration in LPS/HI-injured murine brains by flow cytometry. Representative flow plots (b) and quantification (c) showed increase of CD45^hiCD11b⁺ leukocytes, CD45^hiCD11b⁺Ly6G⁺ neutrophils, and MHC class II⁺ myeloid cells in the brain from 6 to 48 h after LPS/HI injury. Each dot on bar graphs represents an individual animal. Data were shown as mean ± SD. *p < 0.05, **p < 0.01, ***p < 0.001, by one-way ANOVA followed post hoc analysis of Tukey test and unpaired Student’s t test in panels a and c, respectively.