Figure 2.

Detection of neutrophils and neutrophil extracellular traps (NETs) after LPS/HI injury in mouse brains. (a) Western blot and densitometric analysis of neutrophil myeloperoxidase (MPO) expression in the ipsilateral (Right) and contralateral (Left) hemispheres at 24-h post-LPS/HI. Each dot on bar graphs represents an individual animal. UN: untouched naïve mice. Data were shown as mean + SD. *p < 0.05, by unpaired Student’s t test. (b) Shown are anti-myeloperoxidase (MPO, a neutrophil marker) with anti-citrullated histone H3 (H3-cit, a NET marker) or Alexa 594-conjugated isolectin B4 (IB4, blood vessel marker), and the merged images in contralateral and contralateral hemispheres at 24 h and in the ipsilateral hemisphere at 48-h post-LPS/HI. Shown are the same patterns in two independent experiments. Arrows show examples of anti-MPO/H3-cit double-labeled cells. Scale bars: 20 µm.