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Immunostainings of the proteins used in PLA assay. The immunofluorescence of VDAC1, IP3R, GRP75 and FXN were performed as control for PLA technique specificity in the SH-SY5Y and FXN-138.2 cells. (A) Representative images of VDAC either with GRP75 or IP3R. (B) Representative images of FXN either with GRP75 or IP3R.
