Fig. 2.

Effect of rs6423677 genotypes on the relative expression level of immunoglobulin heavy chain transcripts with IGHV3-66. A The proportion of clonotypes with IGHV3-66 among all IGH transcripts displayed by rs6423677 genotype for each immunoglobulin isotype. Ten healthy adult volunteers (upper) and nine KD patients (lower) were analyzed. For KD patients, mean data of proportions at the four evaluation points were plotted. The increasing trend of the proportions according to the number of the C allele at rs6423677 was statistically evaluated with Jonckheere-Terpstra trend test. *P < 0.005, ^†P < 0.01. B Time-course change of the sequence reads proportions of immunoglobulin heavy chain transcripts with IGHV3-66 of acute KD patients. Evaluation was done on four time points including (1) acute phase before receiving IVIG (3–8 illness days), (2) 48 h after the patients became afebrile (8–18 illness days), (3) the first follow-up visit to the pediatric clinic after discharge (17–50 days after the disease onset) and (4) the second follow-up visit to the pediatric clinic after discharge (3–4 months after the disease onset).In each panel, symbols and lines of red, green, and blue colors are representing KD patients with genotypes of AA, AC, and CC at rs6423677, respectively. The analyses were carried out for four immunoglobulin classes (IgM, IgD, IgG, and IgA)