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. 2020 Oct 22;19:1533033820957026. doi: 10.1177/1533033820957026

Figure 5.

Figure 5.

CXCR2 is a functional target of miR-495-3p in glioma cells. A: miR-495-3p contains a potential binding site for CXCR2 as predicted by TargetScan (http://www.targetscan.org/vert_72/). B. RT-PCR was used to detect CXCR2 mRNA level in glioma and normal tissues. C and D. Pearson repression analysis was used to compare the relationships of circ_0000215 and CXCR2 (C), miR-495-3p and CXCR2 (D). E. Dual-luciferase reporter gene assay was used to verify the binding relationship between miR-495-3p and CXCR2. F. Enrichment of CXCR2 in immunoprecipitation of cell lysates were detected by qRT-PCR in RIP assay. G and H: The expression of CXCR2 was detected via qRT-PCR. I and J: The protein level of CXCR2, PI3 K, Akt were detected by western blot. NS, *,**,*** represents P > 0.05, P < 0.05, P < 0.01 and P < 0.001.