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. 2020 Oct 22;8:2050312120966456. doi: 10.1177/2050312120966456

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© The Author(s) 2020

This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (https://creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access page (https://us.sagepub.com/en-us/nam/open-access-at-sage).

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Figure 5. — Directed differentiation into cardiomyocytes using hiPSC at high passages yields positive cells for cardiac markers. (a) Immunostaining of cardiomyocytes (iPSC-CMs ACP5) obtained from ACP5 clone showing protein expression of cardiac specific-markers TNNI1, TNNI3, MYH7, TNNT2, NKX2.5 and ACTN2. Nuclei was stained with Hoechst 3342. (b) and (c) FC data showing protein expression of cardiac-specific markers TNNI1, TNNI3, TNNT2 and ACTN2 in hiPSC-CM. Results were confirmed by performing independent experiments using APC5, PC4.3, PC4.5, PC4.6. Data is presented as mean ± SD, n = 2–5; one-way ANOVA p = 0.9 (ACTN2); 0.2 (TNNI1); 0.9; and 0.1 (TNNT2).