Figure 5.

RTD-1 is able to inhibit furin pre-cleaved (fpc) HPV16 PsV, capacity to inhibit is partially influenced by the HSPG-status of virions, and RTD-1 has cross hrHPV genotype efficacy. Furin pre cleaved (fpc) HPV16 PsV, HPV16 PsV that had been treated with heparinase and trypsin before purification (HSPG-), and hrHPV genotypes 18 and 31 shown. (A) RTD-1 is able to inhibit fpc HPV16 PsV and has a reduced capacity to inhibit HSPG-free HPV16 PsV. Infection with non-treated HPV16 PsV, fpc PsV, or HSPG- PsV were used to normalize infection. Individual points on graph are means of triplicate wells ± SD with a non-linear regression curve. IC₅₀ for each curve are shown. RTD-1 inhibits infection of HeLa cells by both (B) HPV18 and (C) HPV31 high-risk genotypes. Shown is the mean %GFP+ cells of triplicate wells ±SD. Genotype specific antibodies were used for neutralization controls. (C) ^****p < 0.0001, one-way ANOVA followed by Dunnett's multiple comparisons test against PsV-only group.