Fig. 3.

Real-time fluorescence of RT-LAMP (a) and RT-PCR (b) amplification with 10-fold gradually diluted in vitro transcribed RNA as templates. For each template concentration, three repeats are tested simultaneously and one fluorescence curve for each template concentration is shown here. 2 μL of amplified products are gel electrophoresed and imaged shown in the bottom. (c) Visual detection results after RT-LAMP amplified for 5, 10, 15, 20, 25, 30, 35, 40 min, respectively. For each time point, three repeats of samples are photographed at the same time and one represented image is shown here.