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. 2020 Oct 13;23(11):101664. doi: 10.1016/j.isci.2020.101664

Figure 2.

Figure 2

NucSF are Sites of Accumulation of Heterochromatic Markers

(A and B) U2OS cells transiently expressing GFP-RepoMan (green) and treated with 0.5 mM arsenite for 30 min were fixed and stained with anti-HP1γ (A) or anti-H3K9me3 (B) and Alexa Fluor 555 secondary antibodies (red).

(C) Simplified model of ExM protocol. The expansion factor averaged 7.1x (see Figure S4).

(D) Airyscan image of an expanded arsenite-stressed U2OS cell expressing GFP-RepoMan and fixed and stained with anti-H3K9me3-AlexaFluor555 and Hoechst 33342.

(E) 3D volume rendering of the data set in (D).

(F and G) Enlarged insets (F and G) show RepoMan (green) relative to H3K9me3 (red) and DNA (blue). Scale bars are 5 μm.