Table 2.

Summary of iIsor850’s biomass composition.

Constituentsa			Composition (%)
			iIsor850	Yeast7.6
Proteinb			45.18	35.71
l-Alanine	l-Arginine	l-Asparagine
l-Aspartate	l-Cysteine	l-Glutamine
l-Glutamate	Glycine	l-Histidine
l-Isoleucine	l-Leucine	l-Lysine
l-Methionine	l-Phenylalanine	l-Proline
l-Serine	l-Threonine	l-Tryptophan
l-Tyrosine	l-Valine
Carbohydrate			36.80	52.27
1,3-beta-d-Glucan	1,6-beta-d-Glucan	Chitin
N-Glycanc	O-Glycanc	GPI-anchorc
Lipid			5.0	0.74
Episterol	Free fatty acids (6 species)d	Inositol-P-ceramide
Phosphatidylcholine	Phosphatidylethanolamine	Phosphatidylinositol
Phosphatidylserine	TAG
RNAe			8.63	5.85
ATP	CTP	GTP
UTP
DNAe			0.48	0.34
dATP	dCTP	dGTP
dTTP
Cofactors and prosthetic groups			0.06	0.03
S-Adenosyl-L-methionine	Biotin	Coenzyme-A
FAD	Heme A	NAD
NADP	Riboflavin	Spermidine
Tetrahydrofolate	Thiamine diphosphate
Inorganic ions			3.85	5.06
Calcium	Copper	Iron
Magnesium	Manganese	Phosphate
Potassium	Sulfate	Zinc

^aBiomass constituents absent from Yeast 7.6 are underlined. Different representations of Yeast 7.6 biomass constituents are noted below.

^bIdentical to those in yeast 7.6, amino acids in the biomass objective function are in charged-tRNA form, but are listed here as the uncharged amino acids.

^cThe generic mannan (mannose-containing) metabolite in Yeast 7.6 was replaced with three specific essential cell wall components.

^dSix free fatty acid species were abundant (>1% weight) in growth experiments: palmitate (C16:0), palmitoleate (C16:1), 10Z-heptadecenoate (C17:1), oleate (C18:1), linoleate (C18:2) and linolenate (C18:3).

^eMonophosphate ribonucleic and deoxyribonucleic acids present in Yeast 7.6 were replaced with the corresponding triphosphate ones in iIsor850.