Table 3.
List of strains and plasmids used in this study.
| Strains/Plasmids | Description | Sources |
|---|---|---|
| Strains | ||
| E. coli DH5α | Cloning host | NEB |
| I. orientalis SD108 | Wild type | Xiao et al., 2014 |
| SD108ΔUra | URA3Δ, host for plasmids in this study | Xiao et al., 2014 |
| SD108Δ2223 | SD108 with disruption of JL09_g2223 | This study |
| SD108Δ850 | SD108 with disruption of JL09_g850 | This study |
| SD108Δ2279 | SD108 with disruption of JL09_g2279 | This study |
| SD108Δ1386 | SD108 with disruption of JL09_g1386 | This study |
| SD108Δ1702 | SD108 with disruption of JL09_g1702 | This study |
| SD108Δ4163 | SD108 with disruption of JL09_g4163 | This study |
| SD108Δ4262 | SD108 with disruption of JL09_g4262 | This study |
| SD108Δ3351 | SD108 with disruption of JL09_g3351 | This study |
| SD108Δ3078 | SD108 with disruption of JL09_g3078 | This study |
| Plasmids | ||
| pVT15b-epi | CRISPR/Cas9 plasmid, containing ScARS, IoURA3, iCas9, RPR1 promoter, and sgRNA scaffold. Used for cloning of guide RNA and homology arm | Tran et al., 2019 |
| pVT15b-XYZ | pVT15b plasmid carrying the homology arm and guide RNA against any one of these genes: g2223, g850, g2279, g1386, g1702, g4163, g4262, g3351, or g3078 |
This study |