Figure 8.

The LDVBD domain of TarP is sufficient to inhibit cell migration. MEFs that were mock-infected, Chlamydia-infected, vector-only–transfected, or LDVBD-transfected were seeded within ibidi µ-slide live-cell imaging chambers. Time-lapse imaging was performed every 10 min for 10 h to evaluate cell motility. A, for analysis of the infection experiments, a 5-h imaging window common to both mock- and Chlamydia-infected samples was chosen that maximized the number of cells that remained within the field of view. Cells were tracked using the manual tracking function in ImageJ, and the cell trajectory was traced and plotted with the starting points assigned to the origin. B, analysis of the transfection experiment was in a common 10-h imaging window. Data were acquired and plotted as in A. C, velocity and Euclidean distance traveled were calculated for each cell from each experimental group. Values were plotted as dot plots with mean ± S.D. indicated by the bars. Statistical significance was calculated using ANOVA. *, p < 0.01.