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. 2020 Oct 22;14(1):100917. doi: 10.1016/j.tranon.2020.100917

Fig. 1.

Fig. 1

Generation and confirmation of cisplatin resistance in OVCAR8-CP1 and OVCAR8-CP5 cell lines. (A) Schematic depicting the development of the cisplatin-resistant cell lines. (B) Bright-field microscopic images of OVCAR8-CP0, OVCAR8-CP1, and OVCAR8-CP5. Scale bar = 200 μm. (C) All three cell lines were incubated with various concentrations of cisplatin, with a final concentration of 1000 μM cisplatin, for 72 h. Cell viability was determined by the CellTiter-Glo assay system. Values shown represent the mean of eight biological replicates, with the standard error of mean indicated by error bars. (D) IC50 levels are displayed as the mean of eight biological replicates at which% cell survival is at y = 50%. n = 8, **** p < 0.0001. (E) Bright-field microscopic images of OVCAR8-CP0, OVCAR8-CP1, and OVCAR8-CP5 cells 8 days after plating. Scale bar = 10,000 μm (F) Object sum area (μm2) of all plates was determined using BioTek Lionheart FX and is shown as the mean of six biological replicates with the standard error of mean indicated by error bars. n = 6, **** p < 0.0001.