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. 2020 Jul 14;29:0963689720923578. doi: 10.1177/0963689720923578

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© The Author(s) 2020

This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (https://creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).

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Figure 5. — Tregs expanded in the Quantum system suppress proliferation of rat splenocytes. Mean viability for Tregs from the Quantum system, after cryopreservation and reculture (n = 3 donors) (A). In vitro immunosuppression of rat splenocyte proliferation elicited by Tregs from Quantum system expansions. Mean responses of GFP rat splenocytes cocultured with Tregs (n = 3 donors) after 5 d (B) or 13 d (C) postthaw (*P < 0.05, **P < 0.005 by Student’s t-test). Correlation between maximum day 4 in vitro suppression of rat splenocyte proliferation and Quantum system Treg yield (D) and Treg IL-10 stimulation index (E). Fluorescent images of Tregs immunolabeled for CD4, CD25, and FoxP3 (F), scale: 200 (top) and 400 (bottom) µm. Error bars depict s.e.m. CD4: PE; CD25: Alexa 488; DAPI: 4', 6-diamidino-2-phenylindole; FoxP3: forkhead box protein P3: PE; GFP: green fluorescent protein; hTreg: human regulatory T cells; IL-10: interleukin-10; Tregs: regulatory T cells.