Table 2.
Post-expansion Treg Cytokine Secretion After Cryopreservation and Restimulation With Soluble Activator Complex.
| Treg Cell Expansion |
IL-10 Secretion (n = 3) Stimulation Index |
TGF-β1 Secretion (n = 3) Stimulation Index |
|---|---|---|
| Donor 1 | ||
| Flask | 12 | 1 |
| Quantum system | 403 | 1 |
| Donor 2 | ||
| Flask | 1 | 2 |
| Quantum system | 180 | 1 |
| Donor 3 | ||
| Flask | 480 | 1 |
| Quantum system | 3,277 | 1 |
| Mean flask | 164 | 1 |
| s.e.m. | 158 | 0 |
| Mean Quantum system | 1,287 | 1 |
| s.e.m. | 997 | 0 |
The cytokine stimulation indices were normalized for each of the three donor cell products based on the stimulated values (pg/ml) divided by unstimulated values (pg/ml). IL-10: interleukin-10; s.e.m.: standard error of the mean; TGF-β1: transforming growth factor-β1; Tregs: regulatory T cells.