Fig. 2.

Second transplantation of BALB/c and C3H islets to confirm tolerance. (A) Schematic illustration of the experimental setup. After recovery to normoglycemia, second allo-islets were transplanted under the left kidney capsule of the recipients without administration of immunosuppressive drugs. #1 and 2 mice were transplanted with single BALB/c islets (blue line circle), and #3 and 4 mice were transplanted with BALB/c islets (blue line circle) and C3H islets (red dot circle) at different sites of the same kidney. After 34 or 14 d, the islet-bearing kidneys were removed (nephrectomy) and fixed with 4% paraformaldehyde (PFA) for subsequent immunostaining. (B) Immunohistochemical stain of the surviving islets under the kidney capsule. Section slides were triple-stained with anti-CD3 (brown), anti-insulin (red), and anti-FoxP3 (blue). Original magnification 200, 100, and 50 μm.

mAb: monoclonal antibody; STZ: streptozotocin, TPX: transplantation