Fig. 3.
Second transplantation of skin grafts to confirm tolerance. (A) Schematic illustration of the experimental setup. Tail skin grafts were obtained from naïve B6 and BALB/c mice and transplanted into the left flank of (I) tolerant, (II) naïve, and (III) presensitized B6 mice. Presensitized B6 mice were prepared by intraperitoneally injecting irradiated BALB/c splenocytes (7 × 105 cells/mouse). (B) Survival graph of BALB/c skin grafts was plotted. (C) BGL was measured with a OneTouch Ultra device from day 0. The blood was obtained from snipped tail. (D) Immunohistochemical stain of liver tissues of tolerant B6 mice. Section slides were triple-stained with anti-CD3 (brown), anti-insulin (red), and anti-FoxP3 (blue). Original magnification 100 and 50 μm.
STZ: streptozotocin.