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. 2020 May 15;29:0963689720919444. doi: 10.1177/0963689720919444

Fig. 1.

Fig. 1.

Viability assessment using conventional and semi-automated methods. (A) An example of viability calculation using the conventional manual method (green: FDA-positive area; red: PI-positive area). Overall viability is assessed based on estimated viability in individual islets; islets are assessed in the enlarged figure (upper right). Calculation according to the conventional assessment is described. (B) An example of viability calculation using the semi-automated method (blue: FDA-positive area; pink: PI-positive area). Overall viability is assessed based on the total islet area and total dead area of all islets in the micrograph image (not based on individual islet viability). Scale bar: 500 µm.

FDA: fluorescein diacetate; PI: propidium iodide.