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Scoring of Islet Viability in the Conventional Manual Method Using FDA/PI Staining.
| Description | Estimated % viability |
|---|---|
| Almost all cells stained green (FDA-positive); few to no cells stained red (PI-positive) | 100 |
| ≥65% islet cells stained green | 75 |
| 35%-65% islet cells stained green | 50 |
| ≤35% islet cells stained green | 25 |
| Few to no cells stained green | 0 |
FDA: fluorescein diacetate; PI: propidium iodide.
