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Cell culture and optimization of transfection conditions. (A) SKBR3 cell morphology under ×10 magnification by an inverted phase contrast microscope. (B) Green fluorescent protein plasmid transfection into SKBR3 cells using different transfection parameters. (C) Flow cytometry analysis of the transfection efficiency to determine the best transfection conditions. SSC, side scatter; H, height; FL1-H, FL1-height.
