Figure 3.

Hydrogel-integrated multiwell culture chamber for T cell stimulation. (a) Exploded view of the assembly. (b) The interior of the assembly with a detailed view showing how the recessed rectangular slot enabled alignment and fitting of the PDMS gasket. (c) The gasket was created using a PTFE mold and could be easily detached using tweezers (preferably with flat tips). (d) Top and side views show 200 μL of culture medium loaded in each microwell without any leakage. (e) Image of Jurkat cells inside the hydrogel-integrated culture chamber, as viewed from the bottom viewing window using an inverted phase contrast microscope. Scale bar = 100 μm. (f) A gel-coated slide removed from the assembly after aspiration of culture medium from the microwells. Residual medium formed circular droplets on the slide where the microwells were before disassembly. Scale bar = 10 mm.