Table 1. Ingredients for Polyacrylamide Hydrogel Fabrication.
concentration (w/v %) |
volume
from stock (μL) |
||||||
---|---|---|---|---|---|---|---|
acrylamide | bis-acrylamide | streptavidin-acrylamide (×10–3) | 40% (w/v) acrylamide | 2% (w/v) bis-acrylamide | 2 mg/mL streptavidin-acrylamide | 1× PBS | mean stiffness in kPa (see Figure 4a) |
10 | 0.05 | 1.6 | 250 | 25 | 8 | 717 | 7.1 |
10 | 0.1 | 4.0 | 250 | 50 | 20 | 680 | 9.3 |
10 | 0.4 | 70 | 250 | 200 | 350 | 200 | 50.6 |