Table 2. Antibacterial Activity of Lectin Targeted Conjugates 22–31, 20, and Ciprofloxacin (3) against a Panel of Bacterial Organisms. LecA-targeting galactosides were generally more active than the LecB-targeting conjugates. A shorter linker length on the side of the antibiotic led to increased antimicrobial activity^a.

	target: LecA								target: LecB		references
compound	22	23	24	25	26	27	28	29	30	31	20	3
molecular mass [g/mol]	739.8	753.8	753.8	767.8	767.8	781.9	781.9	795.9	761.8	775.9	369.4	331.3
linker length n/m	1/0	1/1	2/0	2/1	3/0	3/1	4/0	4/1	-/0	-/1	0
test organism	MIC [μg/mL]
E. coli K12 MG1655	2	8–16	2	16	1–2	16	2–4	16	8–16	16	n.d.	<0.125
E. coli DSM 1116	2–4	16	2–4	32	2–32	4–32	4–32	4–32	16–32	32	n.d.	<0.125
S. carnosus DSM 20501	32	64	32	>64	16	64	8	≥64	>64	>64	n.d.	<0.125
P. aeruginosa PA14 wt	16	≥64	8–16	>64	8–16	>64	32	>64	64	>64	2–4	0.025–0.1
P. aeruginosa PA14 wt + 1 μg/mL PMBN	4–16	16–64	8–16	32–64	4	32–64	2–8	32–64	64	64	0.025–0.5	0.025
P. aeruginosa PA14 ΔlecA	16–32	≥64	8–16	>64	8–16	>64	32	>64	≥64	>64	4–8	0.05–0.08
P. aeruginosa PA14 ΔlecB	16–32	≥64	8–32	>64	8–16	>64	32–64	>64	64	>64	4	0.05–0.08
P. aeruginosa PAO1 wt	16–32	>64	16	>64	16–32	>64	32–64	>64	≥64	>64	4–8	0.025–0.08
P. aeruginosa PAO1 wt + 1 μg/mL PMBN	4–8	32–64	4–8	32–64	4–8	32–64	8–16	32–64	32–64	≥64	1–2	0.025–0.05

^aData is presented as minimal inhibitory concentration (MIC) range from at least three independent experiments. Molar MIC is given in Table S2. n.d. = not determined.