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. 2020 Sep 18;63(20):11809–11818. doi: 10.1021/acs.jmedchem.0c00958

Figure 4.

Figure 4

Effects of drugs on the gramicidin monomer ↔ dimer equilibrium. (a) Schematic description of the stopped-flow fluorescence quench experiments: gramicidin permeable Tl+ quenches the LUV encapsulated fluorophore ANTS. (b) Representative fluorescence quench traces in DC22:1PC LUVs doped with gD. (c) Effects of drugs on the fluorescence quench rates (gramicidin monomer ↔ dimer equilibrium) in DC18:1PC LUVs doped with gD (light gray), DC22:1PC LUVs doped with gD (dark gray), and DC22:1PC LUVs doped with gA (black). The aqueous drug concentrations were 100, 30, 1800, 10, 300, 30, and 30,000 μM for capsaicin, resveratrol, octanol, C12E6, FC12, Triton X-100, and cyclohexane, respectively, and the estimated molar ratios of the drugs in the bilayers were 0.32, 0.02, 0.48, 0.04, 0.21, 0.04, and 0.93, respectively; cholesterol was added at a molar ratio of cholesterol:lipid of 1:10 when preparing the LUVs. Mean ± S.D. n = 3–4.