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De novo amyloid formation in mixed solutions of A–B and B–A. (A) ThT-fluorescence-monitored kinetics of aggregation of mixed A–B and B–A at various mass ratios at 37 °C/300 rpm. Total peptide concentration was maintained at 0.5 mg/mL. Solution composition: 9 vol/vol % DMSO in 50 mM NaCl, 20 μM ThT, pH 1.9. (B) ATR FT-IR spectra of aggregated samples collected at the end of the kinetic experiment; pellets were initially washed with an excess of 50 mM NaCl, pH 1.9.
