Fig. 3: replication kinetics of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in four different cell lines (Vero E6, Vero CCL-8, Vero hSLAM and HEp-2). Cells were inoculated at a multiplicity of infection (MOI) of 0.02. Virus from the different cell (C) and supernatant (S) were harvested and acquired every 12 h during 96 h post-inoculation (h.p.i.) and quantified by either tissue culture infectious dose (TCID₅₀/mL) and RT-qPCR. Time courses were determined in triplicates, by two independent experiments and the error bars represent standard error of the mean (SEM). (A) Time course quantification of infectious virus titres are indicated the tested cell lines. (B) Quantification of virus RNA copy numbers by RT-qPCR. (C) RNA_cn:TCID₅₀ ratios. RNA_cn: ribonucleic acid copy number.