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. 2020 Aug 24;12(5):plaa042. doi: 10.1093/aobpla/plaa042

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© The Author(s) 2020. Published by Oxford University Press on behalf of the Annals of Botany Company.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 3. — Proteomic workflow: tissues were flash-frozen in liquid nitrogen. Proteins were extracted and digested into fragments using a combined action of trypsin and Lys-C mix. The protein fragments were separated using liquid chromatography (LC) and then quantified by mass spectrometry (MS). The MS/MS spectra were searched against the IWGSC v1.0 genome assembly of wheat (Triticum aestivum) recently published by Apples et al. (2018). Finally, label-free quantitation values were used in log2 scale for t-test comparisons.