Table 7.
Final PCR thermocycler program.
| Temperature (°C) | Time | Cycles |
|---|---|---|
| 95 | 1 min | 1 |
| 95 | 15 s | Optimized cycle number (~15) |
| 65 | 30 s | |
| 68 | 3 min | |
| 68 | 5 min | 1 |
| 4 | hold | -- |
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