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. 2020 Apr 16;62(10):1518–1534. doi: 10.1111/jipb.12925

Figure 5.

Figure 5

Trehalose enhances abscisic acid (ABA)‐induced reactive oxygen species (ROS) accumulation in roots and stomata

(A) Nitroblue tetrazolium chloride (NBT) staining for superoxide anion in the primary roots of wild type (WT), tppe‐1, tppe‐2, 35S:TPPE‐1, and 35S:TPPE‐2 seedlings after ABA treatment. Scale bar, 100 μm. (B) Relative intensities calculated from (A). The values are means ± SD (n > 20). Different letters indicate significant differences at P < 0.05 (one‐way analysis of variance (ANOVA)). (C) H2DCFDA staining for ROS in primary roots of WT, tppe‐1, tppe‐2, 35S:TPPE‐1, and 35S:TPPE‐2 seedlings after ABA treatment. Scale bar, 100 μm. (D) Relative intensities calculated from (C). The values are means ± SD (n > 10). Different letters indicate significant differences at P < 0.05 (one‐way ANOVA). (E) Statistical analysis of the root lengths of seedlings grown on ½ MS medium supplemented with ABA and 100 µM GSH or 2 µM DPI. The values are means ± SD (n > 10). Different letters indicate significant differences at P < 0.05 (one‐way ANOVA).