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. 2020 May 26;235(12):9851–9863. doi: 10.1002/jcp.29797

Figure 1.

Figure 1

miRNA‐204‐5p silencing induces mitochondrial biogenesis through PGC‐1α induction. (a) Representation of the binding between the 3′‐UTR of PGC‐1α mRNA and miRNA‐204‐5p in different species. (b) Activity assay of a luciferase reporter constructs harboring the PGC‐1α 3′‐UTR after transfection with a synthetic precursor for miRNA‐204‐5p. Data shown are the result of three independently executed duplicate experiments. (c) Relative PGC‐1α gene expression upon miRNA‐204‐5p silencing compared to Negative Control A (NegA), 24 and 48 hr posttransfection in C2C12 myotubes. (d) Citrate synthase activity following 24 and 48 hr transfection of anti‐miRNA‐204‐5p versus NegA in C2C12 myotubes. (d) Mitochondrial DNA copy number and nuclear DNA upon miRNA‐204‐5p silencing versus NegA. *p < .05 and **p < .01 (n = 3). 3′‐UTR, 3′‐untranslated region; miRNA, microRNA; mRNA, messenger RNA; PGC‐1α, peroxisome proliferator‐activated receptor‐gamma coactivator‐1α