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R domains for peptide release in engineered NRP biosynthesis. A) Schematic representation of engineered NRPSs with different R domains and peptide production as determined in triplicate. B) Structures of 2 a, 2 b and 2 e. See Figure 1 for assignment of the domain symbols; further symbol: dual condensation/epimerization (C/E; diamond) domain. The colour code at the bottom identifies NRPSs used as building blocks (Figure S9).
