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. 2020 May 20;112(16):1194–1208. doi: 10.1002/bdr2.1709

FIGURE 5.

FIGURE 5

Changes in vitamin A content of the maternal diet alters gene expression in the hindbrain. Expression of Cyp26b1 (a)–(f) and Krox20 (g)–(l) were assessed by whole mount in situ hybridization in the hindbrain of E9.5 LgDel (LD) embryos and WT (WT) littermates (between 17 and 22 somite stages) from dams fed a purified diet containing 4 IU, 10 IU or 16 IU vitamin A as retinol palmitate per gram. (a)–(f) Cyp26b1 is expressed in r5 and r6 and ventrally in r2–r4. Representative images showing increased expression in both LgDel embryos and WT littermates from dams fed the 4 IU diet. Expression is increased further with the 16 IU diet where expression in r4 expands dorsally in LgDel embryos. (g, h) Expression of Krox20 in r5 was reduced in LgDel embryos from dams fed the 10 IU diet and expression was restored with altered maternal vitamin A intake. The number of embryo that presented with the expression pattern shown is indicated as well as the somite stage (so). The otic vesicle is positioned normally adjacent to r5 and r6 in all embryos examined (outlined by the white dotted line)