Fig. 1.

Localisation and activity of YFP‐ZmPIP2;5 expressed in Black Mexican Sweet (BMS) cells. (a) Confocal images of maize BMS cells stably transformed with the 35S:YFP‐ZmPIP2;5 construct. A clear colocalisation of the YFP (green) and the plasma membrane dye FM4‐64 (magenta) was observed. Bars, 10 µm. (b) Water permeability coefficient (P _f) values of nontransformed BMS cells (wild‐type (WT)) and cells expressing YFP‐ZmPIP2;5 (ZmPIP2;5OE). For each scatter plot the lines indicate the mean ± SE. An unpaired t‐test was used to test the statistical difference between lines (***, P < 0.001).