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. 2020 Jul 13;228(3):973–988. doi: 10.1111/nph.16743

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©2020 The Authors. New Phytologist ©2020 New Phytologist Trust

This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.

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Fig. 3 — ZmVAP27‐1 major sperm domain (MSD) is required for the interaction with ZmPIP2;5. (a) Domain composition of the VAP27 proteins that interact with ZmPIP2;5. CCD, coiled‐coil domain; TMD, transmembrane domain. (b) Fluorescence ratiometric quantification from bimolecular fluorescence complementation (BiFC) experiments with the pairs PIP/PMA (YFPn‐ZmPIP2;5/YFPc‐NpPMA2), PIP/VAP (YFPn‐ZmPIP2;5/YFPc‐ZmVAP27‐1), PIP/ΔMSD (YFPn‐ZmPIP2;5/YFPc‐ZmVAP27‐1ΔMSD), PIP/ΔTMD (YFPn‐ZmPIP2;5/YFPc‐ZmVAP27‐1ΔTMD), PIP/ΔCCD (YFPn‐ZmPIP2;5/YFPc‐ZmVAP27‐1ΔCCD), and PIPΔC/VAP (YFPn‐ZmPIP2;5ΔC/YFPc‐ZmVAP27‐1). For each scatter plot, the lines indicate the mean ± SE. The Kruskal–Wallis and Dunn’s multiple comparison test were used to calculate the statistical difference between the negative control and the remaining constructs (***, P < 0.001; *, P < 0.05; ns, not significant). This experiment was repeated twice.