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. 2020 Jul 13;228(3):973–988. doi: 10.1111/nph.16743

Fig. 6.

Fig. 6

ZmVAP27‐1 increases Xenopus oocyte P f when co‐expressed with ZmPIP2;5. (a) Water permeability coefficient (P f) of noninjected oocytes (NI, control) or injected with ZmVAP27:1 cRNA alone (7.5 ng), ZmPIP2;5 cRNA alone (0.75 ng), or coinjected with both cRNAs in different mass ratios (1 : 1 or 1 : 10). For each scatter plot the lines indicate the mean ± SE. ANOVA and Bonferroni’s multiple comparison test were used to calculate the statistical difference between treatments (***, P < 0.001; **, P < 0.01; ns, not significant). The differences reported here were observed with three independent oocyte batches. (b) Relative P f after cytosolic acidification tested in oocytes injected with ZmPIP2;5 cRNA alone (0.75 ng), or with ZmPIP2;5 (0.75 ng) and ZmVAP27:1 (7.5 ng) cRNAs (1 : 10 ratio). Data points are representative values obtained from the same oocyte batch (mean relative P f ± SE). This is one representative experiment of two independent experiments.