Figure 1.

Effects of IRA irradiation on cell proliferation of NHEKs. (a) Cells were counted at the indicated time points after IRA irradiation (800 J cm⁻²). Data are the mean ± standard error (SE) of four independent experiments (n = 4). **P < 0.01 vs control by three‐way ANOVA (group–time interaction at each time). (b) Cells were counted 48 h after IRA irradiation (200–800 J cm⁻²). Data are the mean ± SE of two independent experiments (n = 10). IRA dose‐dependently reduced the number of NHEKs by Williams’ test (*P < 0.05, **P < 0.01 vs control). (c) Representative images of control and IRA‐irradiated cells captured 48 h after IRA irradiation (800 J cm⁻²). Scale bar represents 100 μm. (d) Western blot analysis was performed at the indicated time points (▼) after IRA irradiation (800 J cm⁻²). Representative results from three independent experiments are shown. (e) LDH release was examined 48 h after IRA irradiation (800 J cm⁻²). Data are the mean ± SE of three independent experiments (n = 12). There was no significant difference between control and IRA‐irradiated cells by two‐way ANOVA (Tukey). n.s., not significant.