Figure 4.

IRA irradiation induces sequestration of mTOR into SGs in NHEKs. (a,b) Representative immunofluorescence images of SG formation (a) and colocalization between SGs and mTOR (b) were acquired at the indicated time points after IRA irradiation (200–800 J cm⁻²). (c) Representative Western blot analysis was performed after treatment of NHEKs with LY294002 (10–100 μM) for 1 h (left), and then immunofluorescence images were acquired with or without IRA irradiation (400 J cm⁻²) (right). Scale bar represents 20 μm.