Figure 5.

Sequestration of mTOR into SGs upon IRA irradiation is responsible for mTORC1 inactivation. (a) Representative immunofluorescence images showing colocalization between SGs and mTOR were acquired after IRA irradiation (400 J cm⁻²) with CHX (20 μM) pre‐treatment for 5 min. Scale bar represents 20 μm. (b) After IRA irradiation (400 J cm⁻²), cells were lysed immediately (Pre) or after pre‐treatment with or without CHX (20 μM) followed by 1 mM leucine treatment (Post). Representative results from three experiments are shown. (c) Proposed model of molecular mechanisms involved in the regulation of mTORC1 signaling upon IRA irradiation.