TABLE 1.

Comparisons of limit of detection for V1-V2 16S metagenomics and pan-Borrelia TaqMan PCR

B. burgdorferi DNA level (genomes/ml)	No. of positive samples/total no. of samples
	16S V1-V2 metagenomics			Pan-Borrelia real-time PCR
	B. burgdorferi DNA spiked into blood for B. burgdorferi detection	B. burgdorferi DNA spiked into an Anaplasma-positive blood sample for:		B. burgdorferi DNA spiked into blood for B. burgdorferi detection	B. burgdorferi DNA spiked into an Anaplasma-positive blood sample for B. burgdorferi detection
		B. burgdorferi detection	Anaplasma detection
6 × 106	10/10	10/10	10/10	10/10	10/10
6 × 105	10/10	10/10	10/10	10/10	10/10
6 × 104	10/10	10/10	10/10	10/10	10/10
6,000	10/10	7/10	10/10	9/10	10/10
600	6/10	0/10	10/10	6/10	8/10
60	2/10	0/10	10/10	0/10	0/10