Table 1.
Chemico-physical properties and activity of BSA:B-GLU complex.
| PDI a (S.D.) |
Peak 1 nm ± S.D. (% ± S.D.) |
Peak 2 nm ± S.D. (% ± S.D.) |
Peak 3 nm ± S.D. (% ± S.D.) |
Zpot b mV (S.D.) |
pmol Product/ug B-GLU (Activity at pH 6.7 ± S.D.) | |
|---|---|---|---|---|---|---|
| B-GLU | 0.31 ± 0.04 | 9.5 ± 0.9 (78 ± 12) |
144 ± 45 (24 ± 14) |
/ | 16662 ± 800 | |
| BSA solution (0.15 nM) | 0.28 ± 0.01 | 3.1± 0.1 (77 ± 3) |
19 ± 3 (18 ± 2) |
102 ± 25 (5 ± 2) |
−20 ± 2 | |
| BSA solution (0.75 nM) | 0.33 ± 0.02 | 2.7 ± 0.4 (56 ± 9) |
32 ± 7 (45 ± 11) |
/ | −19 ± 5 | |
| BSA solution (1.5 nM) | 0.38 ± 0.03 | 2.4 ± 0.4 (61 ± 12) |
38 ± 3 (43 ± 7) |
/ | −13 ± 3 | |
| BSA/B-GLU 2:1 mol mol | 0.34 ± 0.01 | 3.5 ± 0.3 (46 ± 8) |
288 ± 34 (53 ± 12) |
−16 ± 1 | 16253 ± 775 | |
| BSA/B-GLU 10:1 mol mol | 0.51 ± 0.04 | 2.5 ± 0.5 (25 ± 11) |
62 ± 6 (48 ± 14) |
241 ± 45 (25 ± 13) |
−18 ± 4 | 16588 ± 675 |
| BSA/B-GLU 20:1 mol mol | 0.62 ± 0.05 | 2.3 ± 0.4 (16 ± 12) |
73 ± 5 (42 ± 12) |
369 ± 68 (41 ± 7) |
−16 ± 3 | 17532 ± 943 |
a: Polydispersity index. b: Zeta potential measurement of nanoparticle surface charge.