Table 2.
Gradient system used to isolate compounds on preparative HPLC.
| Time (min) | Mobile Phase Proportion (%) | |
|---|---|---|
| Solvent A | Solvent B | |
| 0.0 | 0.0 | 100 |
| 2.0 | 3.0 | 97.0 |
| 35.0 | 15.5 | 84.5 |
| 55.0 | 19.0 | 81.0 |
| 76.0 | 27.5 | 72.5 |
| 98.0 | 31.5 | 68.5 |
| 133.0 | 60.0 | 40.0 |
| 144.0 | 60.0 | 40.0 |
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