FIG 7.

Identification of rF- and rFG-repeat binding sites within Fn type III repeat 12. (A) One-amino-acid overlapping 10-mers were immobilized on a microchip. The surface was then probed with fluorescence labeled rF- and rFG-repeats. Both recombinant Embp fragments demonstrated binding to almost the same peptides as shown in the heatmap. (B) Mapping of rF- and rFG-binding peptides onto the amino acid sequence of FN12. Arrows indicate seven β-sheets of FN12 (A to G) (32). The red underlined sequence indicates a projection of peptides with rF- and rFG-repeat binding activity located within the C β-sheet (aa 33 to 43). The blue underlined sequence indicates a projection of peptides with rF-repeat and rFG-repeat binding activity located within the F β-sheet (aa 70 to 79). The amino acid numbering refers to the FN12 sequence, as outlined in Sharma et al. (32).