TABLE 2.

Cell lines, strains, and bacteriophages used in this study

Cell line or bacterial strain	Properties/genetics	Source or reference
Cell line
HEK293	Adherent embryonic kidney cell line for transfection and isolation of Fn and Fn isoforms.	74
Bacterial strains
E. coli TOP10	Cloning host; genotype F– mcrA Δ(mrr-hsdRMS-mcrBC) ϕ80lacZΔM15 ΔlacX74 recA1 araD139 Δ(araleu)7697 galU galK rpsL (Strr) endA1 nupG	Invitrogen, Carlsbad, CA
E. coli BL21 AI	Host for recombinant protein expression; genotype F– ompT hsdSB(rB– mB–) gal dcm araB::T7RNAP-tetA	Invitrogen, Carlsbad, CA
E. coli BL21 STAR	Host for recombinant protein expression; genotype F– ompT hsdSB (rB– mB–) galdcmrne131 (DE3)	Invitrogen, Carlsbad, CA
T7 Express Crystal E. coli	Methionine auxotrophic host for recombinant expression of the selenomethionine-derivatized rF-repeat; genotype: fhuA2 lacZ::T7 gene1 [lon] ompT gal sulA11R(mcr-73::miniTn10−TetS)2 [dcm] R(zgb-210::Tn10−TetS) endA1 metB1 Δ(mcrC-mrr)114::IS10	New England Biolabs, Ipswich, MA
S. epidermidis 1585	Clinical S. epidermidis wild-type isolate from a port catheter infection; icaADBC and aap negative; biofilm negative	22, 75
S. epidermidis 1457-M12	Tn917 insertion in purR; S. epidermidis recipient for plasmid electroporation	76
S. epidermidis 1585Δembp	Markerless embp knockout mutant derived from S. epidermidis 1585	This study
S. epidermidis 1585Pxyl/tetembp	Derivative of S. epidermidis in which embp is placed under the control of an anhydrotetracycline-inducible element	22
S. aureus RN4220	Restriction-deficient mutant derived from S. aureus RN450	77
S. carnosus TM300	Surrogate host	78
ΦA6C	Transduction from/into S. epidermidis strains	53