TABLE 4.
Oligonucleotides used in this study
| No. | Oligonucleotide | Sequence (5′–3′) |
|---|---|---|
| 1 | for_attP | GGGGACAAGTTTGTACAAAAAAGCAGGCTTAACATTTTTATGCAACAAG |
| 2 | 5rev_Pst | GCTACATCTGCAGCAATTTATTTGTTCTAAACAATAATATCAC |
| 3 | 3for_Pst | ATGTAGCCTGCAGTTAGAAAAAGTCGAGCATGCTC |
| 4 | 3rev_attP | GGGGACCACTTTGTACAAGAAAGCTGGGTAGGATTGAATGAATATCCT |
| 5 | embp_−255_for | CCGAAGTGCTTGTGCG |
| 6 | embp_+510_rev | CCGAAGTGCTTGTGCG |
| 7 | inf_Eco_Embp_for | GGTACCGAGCTCGAATTCAAATGCTATTGTGATAAATGAAGAG |
| 8 | inf_Asc_Embp_rev | TGCATTTAGAATAGGCGCGCCATATATTTTACTTTTTAGAAC |
| 9 | 1F_fwd | TGCATCATCATCATCATCACGTGGAAAACCTGTATTTTCAGGGCACTAAAGTTAATAAAACCGAATTAATC |
| 10 | 1F_rev | AATATCATCGATCTCGAGCGTTAGTTGCTTTTAGCTTCAAC |
| 11 | 2F_rev | TTAATTACTTTTAGCATTAGTTAAAGCTTG |
| 12 | 4F_rev | AATATCATCGATCTCGAGCGTTAATGTTGTTTTGCTTCG |
| 13 | 1FG_for | CACCGGAGATCAAAAACTTCAAGATGC |
| 14 | 1FG_rev | TTAATGAAGATTTTGTTCAGCAT |
| 15 | 2FG_rev | TTACAATGAATCTTTTGCTTGAATGA |
| 16 | 5FG_for | CACCGGAGAATCCAGATTTAAACA |
| 17 | 5FG_rev | TTAATGTAAACTTTCTCTAGCATTTTGC |