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. 2020 Oct 20;11(5):e02645-20. doi: 10.1128/mBio.02645-20

FIG 2.

FIG 2

1-TbAd production does not influence the in vitro growth characteristics and behaviors of M. kansasii. (a) Comparative OD600 growth kinetics of WT M. kansasii (MKAN), M. kansasii::EV (MKAN::EV), and M. kansasii::Rv3377-78c (MKAN::Rv33778c) at 37°C in 7H9 broth. The data are presented as the means of technical triplicates ± standard deviations (SD) (error bars). The data are representative of three independent experiments. (b) Colony morphology in two different incubation settings of M. kansasii::EV (MKAN::EV) and M. kansasii::Rv3377-78c (MKAN::Rv33778c) on 7H10 plates. (c) Colony morphology of M. kansasii::EV (MKAN::EV) and M. kansasii::Rv3377-78c (MKAN::Rv33778c) on 7H10 plates supplemented with Congo red. (d) Quantitative analysis of Congo red dye retention by M. kansasii::EV (MKAN::EV) and M. kansasii::Rv3377-78c (MKAN::Rv33778c). DMSO extraction was followed by absorbance at 488 nm divided by the weight of the dry culture pellet after washing (in grams). The data are plotted as the mean of technical triplicates ± SD. The data are representative of two independent experiments.