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. 2020 Oct 20;11(5):e02645-20. doi: 10.1128/mBio.02645-20

FIG 6.

FIG 6

1-TbAd enhances the initial establishment of pulmonary infection. (a and b) CFU were counted from C57BL/6 mouse lungs isolated at 4 h versus 24 h after aerosol infection with M. kansasii::EV (MKAN::EV) or M. kansasii::Rv3377-78c (MKAN::Rv33778c). (a) Absolute CFU count data are pooled from two independent experiments with similar initial inocula (n = 16 to 20 lungs/condition/time point). (b) 24-h CFU/mean 4-h CFU ratio data were pooled from three independent experiments (n = 21 to 25 lungs/condition/time point). (c to e) CFU were counted from C57BL/6 murine-derived alveolar macrophages (AMs) at 4 and 24 h after ex vivo M. kansasii infection. (c and d) Absolute CFU count data from two independent experiments (n = 5 to 7 replicate wells containing infected AMs, respectively, per condition per time point). (e) 24-h CFU/mean 4-h CFU ratio data pooled from two independent experiments (n = 12 per condition). The data are plotted as the means ± SD. GraphPad Prism 8.1.2 was used to perform Welch’s two-tailed unpaired t tests where statistical significance is indicated by asterisks as follows: *, P < 0.05; **, P < 0.01; ****, P < 0.0001.