FIG 8.

TgStx12 is essential for protein transport into the apicoplast. (A) Electron microscopy reveals no gross morphological defects of the micropore (indicated with an arrowhead) in parasites depleted in Stx12. (B) Apicoplast is shown to be lost in a subpopulation of Stx12 cKD parasites upon 72 hours of treatment with rapamycin. Representative images are shown. Cpn60 or ATrx were used as apicoplast markers. Scale bars = 7 μm. (C) Quantification of apicoplast upon depletion of Stx12 for 72 hours with rapamycin. Error bars represent the ± SD for three independent experiments. (D) Pro-Cpn60 (marked with a black arrowhead) accumulates upon depletion of Stx12 for 72 hours with rapamycin. Processed Cpn60 is marked with a read arrowhead. (E) Electron microscopy reveals no gross morphological defects of the apicoplast (indicated with an arrow) in parasites depleted in Stx12. Scale bars in A and E = 1 μm.