In vivo biostimulant assays. The biostimulant effect of chitosan hydrochloride at 0.5% was tested on Marco Aurelio and DBC480 by evaluating the kernels germination, number of developed roots, main root length, coleoptile length, and Nitrogen Balance Index (NBI). Results are expressed as % of growth inhibition. The data represent averages and standard errors from three independent experiments organized as a complete randomized block, each one consisting of 20 plants for the experimental group (wheat genotype × compound). The statistical analysis was performed using one-way analysis of variance (ANOVA); “ns” refers to “not significant”.